molecular characterization of subunit g of the vacuolar atpase in pathogen dermatophyte trichophyton rubrum

trichophyton rubrum is an anthropophilic fungus causing up to 90% of chronic cases of dermatophytosis. several properties of this fungus have been investigated so far. however, a few studies were carried out in the field of molecular biology of this fungus. in the present study, we tried to identify the subunit g of its vacuolar atpase (v-atpase). pairs of 21 nt primers were designed from highly conserved regions of the v-atpase subunit g genes in other fungi. mentioned primers were utilized in pcr using isolated genomic dna template as well as cytoplasmic rna of t.rubrum and the pcr and rt-pcr fragments were then sequenced. about 469 nucleotides were sequenced which encoded a polypeptide with 119 amino acids. nucleotide sequence comparison in gene data banks (ncbi, nih) for both the dna and its deduced amino acid sequence revealed significant homology with v-atpase subunit g genes and proteins of other eukaryotic cells. the amino acid sequence of the encoded protein was about 84% identical to the sequence of v-atpase subunit g from other fungi. in summary, we have cloned the first v-atpase subunit g of dermatophytes and characterized it as a member of this gene family in other eukaryotic cells.